rabbit polyclonal  (Proteintech)

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: STIM1 immunohistochemical expression in the studied groups. ( a ) A case of normal pancreatic tissue showed weak cytoplasmic STIM1 epithelial expression in the pancreatic duct and focal cytoplasmic STIM1 stromal expression (IHC x200), ( b ) A case of adjacent non-tumor pancreatic tissue showed moderate cytoplasmic STIM1 epithelial expression and focal STIM1 cytoplasmic stromal expression (IHC x100), ( c ) A case of control non-tumor intestinal tissue showed moderate cytoplasmic STIM1 epithelial expression and focal cytoplasmic STIM1 stromal expression (IHC x100), ( d ) A case of primary PDAC showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100), ( e ) A case of metastatic PDAC to the liver (on the left side) showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100), ( f ) A case of AAC showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100)

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Immunohistochemical staining, Expressing, Control

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Comparison between STIM1 and SOX2 expression in PDAC and the control groups. ( a ) Comparison between STIM1 epithelial expression in PDAC and the control groups, ( b ) Comparison between STIM1 stromal expression in PDAC and the control groups, ( c ) Comparison between SOX2 epithelial expression in PDAC and the control groups, ( d ) Comparison between SOX2 stromal expression in PDAC and the control groups. ( e ) Comparison between STIM1 epithelial expression in AAC and the control groups. ( f ) Comparison between STIM1 stromal expression in AAC and the control groups. ( g ) Comparison between SOX2 stromal expression in PDAC and the control groups. ( h ) Comparison between SOX2 stromal expression in AAC and the control groups

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Comparison, Expressing, Control

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Relationship between STIM1 expression and clinicopathological data in the primary PDAC group (No = 48) and AAC (No = 21)

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Kaplan-Meier survival curve demonstrating the impact of clinicopathological and STIM1/SOX2 expressions on the overall survival of AAC cases

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Univariate and multivariate COX regression analysis for the parameters affecting morality in the primary AAC group

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Selective Assembly of TRPC Channels in the Rat Retina during Photoreceptor Degeneration

doi: 10.3390/ijms25137251

Figure Lengend Snippet: Features of the primary antibodies.

Article Snippet: STIM1 , N-terminal of human STIM1 , Rabbit Polyclonal , Proteintech (Manchester, UK), #11565-1-AP , IC: 1:400.

Techniques: Isolation

Journal: Medicine International

Article Title: Stormorken syndrome caused by STIM1 mutation: A case report and literature review

doi: 10.3892/mi.2022.54

Figure Lengend Snippet: Genetic analysis of STIM1. (A) Information on the pedigree and Sanger sequencing results. The mutation was present in affected patient and absent in the unaffected parents, indicating that it was a spontaneous mutation. (B) Schematic representation of the STIM1 domains. The arrow indicates the position of the substitution in the EF-hand domains. (C) Amino acid sequence conservation of STIM1. The affected amino acids have been highly conserved during evolution. (D) 3D structures of STIM1 and mutated proteins. (E) STIM1 exhibited a comparable level compared to the control in vitro , as indicated using western blot analysis. STIM1, stromal interaction molecule 1; EF, EF-hand domain; TM, transmembrane domain; CC, coiled-coil domain; SP, serine- and proline-rich domain; K, polylysine.

Article Snippet: The membrane was blocked at 4˚C overnight with 5% skim milk, and incubated with primary antibodies at room temperature for 1 h, and then incubated with secondary antibodies at room temperature for 1 h. STIM1 rabbit polyclonal antibody (diluted 1:1,000, cat. no. A19894, ABclonal Biotech Co., Ltd.) was used as a primary antibody.

Techniques: Sequencing, Mutagenesis, In Vitro, Western Blot

Journal: Medicine International

Article Title: Stormorken syndrome caused by STIM1 mutation: A case report and literature review

doi: 10.3892/mi.2022.54

Figure Lengend Snippet: Summary of mutation sites reported in previous studies ( 1 , 4 , 8 , 9 , 11-24 ).

Article Snippet: The membrane was blocked at 4˚C overnight with 5% skim milk, and incubated with primary antibodies at room temperature for 1 h, and then incubated with secondary antibodies at room temperature for 1 h. STIM1 rabbit polyclonal antibody (diluted 1:1,000, cat. no. A19894, ABclonal Biotech Co., Ltd.) was used as a primary antibody.

Techniques: Mutagenesis

Journal: Blood Advances

Article Title: CIC-39Na reverses the thrombocytopenia that characterizes tubular aggregate myopathy

doi: 10.1182/bloodadvances.2021006378

Figure Lengend Snippet: In vivo effect of CIC-39Na in WT and KI-STIM1 I115F mice. (A) Study design of in vivo treatments in WT and KI-STIM1 I115F mice. (B) Platelet count in the indicated groups. Mann-Whitney U test P value: *0.0334 for each experimental point vs WT vehicle. P value: #0.0154, ##0.016; ###0.0001 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. (C) Circulating number of white blood cells and erythrocytes. (D) Absorbance values relative to blood lost in the indicated groups. Mann-Whitney U test. P value: ^^0.003, ^0.0208 for WT vehicle vs KI-STIM1 I115F vehicle and #0.0208 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. (E) Bleeding time in mice treated as indicated. P value: ^0.0208 for WT vehicle vs KI-STIM1 I115F vehicle, #0.0304 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. Data reported in panels B-E represent the mean ± SEM of 8 animals for each group from 2 independent experiments.

Article Snippet: Sections were then incubated in bovine serum albumin (BSA) 2% and then with a primary polyclonal rabbit antibody to STIM1 (#4916 from Cell Signaling) for 1 hour.

Techniques: In Vivo, MANN-WHITNEY

Journal: Blood Advances

Article Title: CIC-39Na reverses the thrombocytopenia that characterizes tubular aggregate myopathy

doi: 10.1182/bloodadvances.2021006378

Figure Lengend Snippet: Progenitor characterization of WT and KI-STIM1 I115F mice. (A-B) Frequency of the indicated progenitor cells; Mann-Whitney U test (n = 6 per group) P value: ^^≤0.0079 and ^≤0.00224 for WT vehicle vs KI-STIM1 I115F vehicle. (C) Representative immunofluorescence staining for STIM1 (green), CD41 (blue), DAPI (cyano), and merged image on megakaryocytes of WT mice (see Supplemental Methods for the other groups). Top panel: Magnification 40 × 1.4 NA (numerical aperture). Bar = 25 μm. Bottom panel: Magnification 63 × 1.4 NA (numerical aperture). Bar = 10 μm. The data are representative of 5 experiments with similar results. (D) Box-and-whisker plots show median and IQR of the mean number of MK colonies/well in WT and KI-STIM1 I115F mice. Mann-Whitney U test. P value: ^≤0.0111 WT vehicle vs KI-STIM1 I115F vehicle.

Article Snippet: Sections were then incubated in bovine serum albumin (BSA) 2% and then with a primary polyclonal rabbit antibody to STIM1 (#4916 from Cell Signaling) for 1 hour.

Techniques: MANN-WHITNEY, Immunofluorescence, Staining, Whisker Assay

Journal: Blood Advances

Article Title: CIC-39Na reverses the thrombocytopenia that characterizes tubular aggregate myopathy

doi: 10.1182/bloodadvances.2021006378

Figure Lengend Snippet: Clearance, STIM1 protein levels and Ca 2+ -signaling in WT and KI-STIM1 I115F platelets. (A) Platelet lifespan in the indicated groups (n = 6). Mann-Whitney U test (n = 6 per group) P value: ^^^≤0.0015 and ^^≤0.0028 for WT vehicle vs KI-STIM1 I115F vehicle; ##≤0.003 and #≤0.0014 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. (B) Representative images and densitometry analysis of western blots for STIM1 and ORAI1 (n = 3); unpaired t test for STIM1 blot ( P value: ****<0.0001 WT vehicle vs WT CIC-39Na; ###<0.0002 KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na and ^^^0.009 WT vehicle vs KI-STIM1 I115F vehicle). ORAI1 blot ( P value: ****<0.0001 WT vehicle vs WT CIC-39Na; ####<0.0001 KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na). (C) Basal platelet cytosolic Ca 2+ . Values are median ± SEM of at least 300 platelets from 2 experimental days. Mann-Whitney U test (n = 6 per group) P value: ^^^^<0.0001 WT vehicle vs KI-STIM1 I115F vehicle and ####<0.0001 KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. (D) Average Ca 2+ -traces depicting tBHQ release and SOCE. Statistical analysis of the AUC of the first 200 seconds after the respective addition are present in the insets. Arrows indicate addition of the SERCA poison in Ca 2+ -free conditions and the readdition of Ca 2+ . Traces are average of 300 cells from 2 different experimental days and color coding is as in panel C; to allow comparisons, F/Fo was offset to 0 in the graphs for all traces. Mann-Whitney U test. P value: ^^^^<0.0001 for WT vehicle vs KI-STIM1 I115F vehicle; ###<0.001 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. (E-F) Average Ca 2+ traces induced by the indicated agonists in Ca 2+ -containing media. Traces are average of 300 cells from 2 different experimental days; statistical analysis of the AUC of the first 200 seconds after the respective addition are present in the insets and color coding is as in panel C; Mann-Whitney U test. P value: ****<0.0001 and **<0.003 for KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na; ^^^<0.001 for WT vehicle vs KI-STIM1 I115F vehicle. To allow visual comparisons, F/Fo was offset to 0 in the graphs for all traces.

Article Snippet: Sections were then incubated in bovine serum albumin (BSA) 2% and then with a primary polyclonal rabbit antibody to STIM1 (#4916 from Cell Signaling) for 1 hour.

Techniques: MANN-WHITNEY, Western Blot

Journal: Blood Advances

Article Title: CIC-39Na reverses the thrombocytopenia that characterizes tubular aggregate myopathy

doi: 10.1182/bloodadvances.2021006378

Figure Lengend Snippet: Platelet function WT and KI-STIM1 I115F (A-B) P-selectin (A) and integrin αIIbβ3 exposure after 10-minute stimulation with the indicated stimuli. (C) platelet spreading to collagen in static conditions, indicated as are of adherent cells (left), and number of adhered platelets (right); BSA was used as negative control in place of collagen. (D) Thrombus formation under flow, indicated as percentage of platelet covered area; Mann-Whitney U test (n = 6 per group) P value: ^^^≤0.0002, ^^≤0.0039 WT vehicle vs KI-STIM1 I115F vehicle; *****<0.00001 WT vehicle vs WT CIC-39Na and ##≤0.0036 KI-STIM1 I115F vehicle vs KI-STIM1 I115F CIC-39Na. Supplemental Figure 4A-B depicts fluorescent microscope images of the results; (E) % of aggregated platelets in the indicated conditions. To reach similar aggregations, WT platelets were diluted 1:3. Supplemental Figure 4C depicts slopes.

Article Snippet: Sections were then incubated in bovine serum albumin (BSA) 2% and then with a primary polyclonal rabbit antibody to STIM1 (#4916 from Cell Signaling) for 1 hour.

Techniques: Negative Control, MANN-WHITNEY, Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Enhanced Orai1-mediated store-operated Ca 2+ channel/calpain signaling contributes to high glucose-induced podocyte injury

doi: 10.1016/j.jbc.2022.101990

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-STIM1 , Proteintech , Cat#: 11565-1-AP.

Techniques: Western Blot, Imaging, Staining, Plasmid Preparation, Activity Assay, Cell Culture